Refereed Papers (from 2014)
- Maruyama K, Tsujimoto Y, Kishimoto M, Yoshiwara K, Sakai H, Sakurai T, Sakamoto S, Mitsuda N, Maruyama-Nakashita A
Identification and characterization of low-sulfur-inducible genes and pathways in roots of upland and lowland rice grown under hydroponic and soil conditions.
J. Exp. Bot. (2026) doi: 10.1093/jxb/erag001 - Zhang J, Liu Z, Farrar EJ, Li M, Lu H, Qu Z, Chara O, Mitsuda N, Sakamoto S, Xue F, Shan Q, Yu Y, Li J, Zhu X, Zhu M, Shi J, Peralta Ogorek L, Borges A, Bennett MJ, Liang W, Pandey BK, Zhang D, Persson S.
Ethylene modulates cell wall mechanics for root responses to compaction.
Nature (2025) doi: 10.1038/s41586-025-09765-7 - Sato Y, Hosaka F, Niki T, Osawa H, Yoshihara E, Sakamoto S, Mitsuda N, Teramura Y
Promoting plant protoplast fusion via membrane decoration with cell-penetrating peptide-conjugated lipids.
Biochem. Biophys. Res. Commun. (2025) doi:10.1016/j.bbrc.2025.152635 - Hamdayanty, Idehara K, Sekine KT, Atsumi G, Sekine R, Tateda C, Yaeno T, Kaya H
A Tradeoff Between the Escape from N′-Mediated Resistance and Virulence in Pepper Mild Mottle Virus Through Reduced Virus Accumulation.
Plants 14: 2471 (2025) doi: 10.3390/plants14162471 - Matsuo K.
Recovery of RNA-dependent RNA polymerase 6 gene-knockout phenotypes in Nicotiana benthamiana via in vivo generation of inverted repeat construct of the trans-acting short interference RNA3 sequence
Plant J. 123 :e70350 (2025) doi: 10.1111/tpj.70350
（＃プレスリリース → 機能性タンパク質を大量に生産できる植物を開発） - Mukai Y, Taguchi Y, Matsuo K
Transient expression of human interleukin-15 in Nicotiana benthamiana.
Plant Biotechnol. 25:0711a. (2025) doi: 10.5511/plantbiotechnology.25.0711a - Atsumi G, Fukuzawa N, Matsumura T
The efficient systemic movement of cucumber mosaic virus vector conferred by 2b and its application for virus-induced gene silencing vector in potato
J. Gen. Plant Pathol. 91: 292–297 (2025) doi: 10.1007/s10327-025-01233-4 - Ohama N, Moo TL, Chung K, Mitsuda N, Boonyaves K, Urano D, Chua NH
MEDIATOR15 destabilizes DELLA protein to promote gibberellin-mediated plant development
New Phytol. (2025) doi: 10.1111/nph.20397 - Surina S, Yamagami A, Miyaji T, Chagan Z, Chung K, Mitsuda N, Nishida K, Tachibana R, Zhu Z, Miyakawa T, Shinozaki K, Sakuta M, Asami T, Nakano T
BIL9 promotes both plant growth via BR signaling and drought stress resistance by binding with the transcription factor HDG11
Plant Cell Physiol. 65: 1640-1654 (2024) doi: 10.1093/pcp/pcae009 - Suzuki H, Sugano SS, Muranaka T, Seki H
Ectopic expression of BpbHLH9 suggested the presence of a self-activating loop mechanism of clade Ia bHLHs to enhance betulinic acid biosynthesis in Lotus japonicus hairy roots
Plant Biotechnol. 41: 319-323 (2024) doi: 10.5511/plantbiotechnology.24.0717b - Hayakawa T, Suzuki H, Yamamoto H, Mitsuda N
Synthetic biology in plants
Plant Biotechnol. 41: 173-193 (2024) doi: 10.5511/plantbiotechnology.24.0630b - Fukuzawa N, Matsuo K, Atsumi G, Tasaka Y, Mitsuda N
Plant-made pharmaceuticals
Plant Biotechnol. 41: 243-260 (2024) doi: 10.5511/plantbiotechnology.24.0716a - Yoshida K, Sakamoto S, Mitsuda N
Synthetic-biology approach for plant lignocellulose engineering
Plant Biotechnol. 41: 213-230 (2024) doi: 10.5511/plantbiotechnology.24.0630a - Nakamura A, Yamamoto H, Yano T, Hasegawa R, Makino Y, Mitsuda N, Terakawa T, Ito S, Sugano SS
Expanding the genome-editing toolbox with Abyssicoccus albus Cas9 using a unique protospacer adjacent motif sequence
CRISPR J. 7: 197-209 (2024) doi: 10.1089/crispr.2024.0013 - Maeo K, Nakaya Y, Mitsuda N, Ishiguro S
ACRE, a class of AP2/ERF transcription factors, activates the expression of sweet potato ß-amylase and sporamin genes through the sugar-responsible element CMSRE-1
Plant Mol. Biol. 114: 54 (2024) doi: 10.1007/s11103-024-01450-z - Ezura K, Lu Y, Suzuki Y, Mitsuda N, Ariizumi T
Class II knotted-like homeodomain protein SlKN5 with BEL1-like homeodomain proteins suppresses fruit greening in tomato fruit
Plant J. 118: 2037-2054 (2024) doi: 10.1111/tpj.16727 - Wu YC, Yu CW, Chiu JY, Chiang YH, Mitsuda N, Yen XC, Huang TP, Chang TF, Yen CJ, Guo WJ
The AT-hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2-mediated sugar retrieval in Arabidopsis roots
Plant Cell Environ. 47: 1084-1098 (2024) doi: 10.1111/pce.14779 - Adhikari PB, Liu XY, Huang C, Mitsuda N, Notaguchi M, Kasahara RD
Transcription factors behind MYB98 regulation: What does the discovery of SaeM suggest
Plants 13: 1007 (2024) doi: 10.3390/plants13071007 - Im JH, Son S, Kim WC, Kim K, Mitsuda N, Ko JH, Han KH
Jasmonate activates secondary cell wall biosynthesis through MYC2-MYB46 module
Plant J. 117: 1099-1114 (2024) doi: 10.1111/tpj.16541 - Todaka D, Quynh DN, Tanaka M, Utsumi Y, Utsumi C, Ezoe A, Takahashi S, Ishida J, Kusano M, Kobayashi M, Saito K, Nagano AJ, Nakano Y, Mitsuda N, Fujiwara S, Seki M
Application of ethanol alleviates heat damage to leaf growth and yield in tomato
Front. Plant Sci. 15: 1325365 (2024) doi: 10.3389/fpls.2024.1325365 - Matsushima A, Matsuo K
Removal of plant endogenous proteins from tobacco leaf extract by freeze-thaw treatment for purification of recombinant proteins
Plant Sci. 339: 111953 (2024) doi: 10.1016/j.plantsci.2023.111953 - Adhikari PB, Zhu S, Liu X, Huang C, Xie L, Wu X, He J, Mitsuda N, Peters B, Brownfield L, Nagawa S, Kasahara RD
Discovery of a cis-regulatory element SaeM involved in dynamic regulation of synergid-specific MYB98
Front. Plant Sci. 14: 1177058 (2023) doi: 10.3389/fpls.2023.1177058 - Isogai M, Yoshikoshi M, Seki K, Masuko-Suzuki H, Watanabe M, Matsuo K, Yaegashi H
Seed transmission of raspberry bushy dwarf virus is blocked in Nicotiana benthamiana plants by preventing virus entry into the embryo from the infected embryo sac and endosperm
Arch. Virol. 168: 138 (2023) doi: 10.1007/s00705-023-05767-w - Nagahage ISP, Matsuda K, Miyashita K, Fujiwara S, Mannapperuma C, Yamada T, Sakamoto S, Ishikawa T, Nagano M, Ohtani M, Kato K, Uchimiya H, Mitsuda N, Kawai-Yamada M, Demura T, Yamaguchi M
NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence
Plant Direct 7: e529 (2023) doi: 10.1002/pld3.529 - Nakamura A, Yano T, Mitsuda N, Furubayashi M, Ito S, Sugano SS, Terakawa T
The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice
Sci. Rep. 13: 14205 (2023) doi: 10.1038/s41598-023-40433-w - Saito M, Momiki R, Ebine K, Yoshitake Y, Nishihama R, Miyakawa T, Nakano T, Mitsuda N, Araki T, Kohchi T, Yamaoka, S
A bHLH heterodimer regulates germ cell differentiation in land plant gametophytes
Curr. Biol. 33: 4980-4987 (2023) doi: 10.1016/j.cub.2023.09.020 - Zhang LM, Kamitakahara H, Takano T, Morimoto T, Sakamoto S, Mitsuda N, Itai, A
Stone cell formation in the pedicel of pears and apples
Planta 258: 85 (2023) doi: 10.1007/s00425-023-04240-x - Atsumi G, Naramoto S, Nishihara M, Nakatsuka T, Tomita R, Matsushita Y, Hoshi N, Shirakawa A, Kobayashi K, Fukuda H, Sekine KT
Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta
J Virol. (2023) doi: 10.1128/jvi.00463-23 - Oda-Yamamizo C, Mitsuda N, Milkowski C, Ito H, Ezura K, Tahara K
Heterologous gene expression system for the production of hydrolyzable tannin intermediates in herbaceous model plants
J. Plant Res. (2023) doi: 10.1007/s10265-023-01484-2 - Huang CK, Kurotani KI, Tabata R, Mitsuda N, Sugita R, Tanoi K, Notaguchi M
Nicotiana benthamiana XYLEM CYSTEINE PROTEASE genes facilitate tracheary element formation in interfamily grafting
Horticulture Res. 10: uhad072 (2023) doi: 10.1093/hr/uhad072 - Lin LY, Chow HX, Chen CH, Mitsuda N, Chou WC, Liu TY
Role of autophagy-related proteins ATG8f and ATG8h in the maintenance of autophagic activity in Arabidopsis roots under phosphate starvation
Front. Plant Sci. 14 (2023) doi: 10.3389/fpls.2023.1018984 - Zhang YL, Maruyama D, Toda E, Kinoshita A, Okamoto T, Mitsuda N, Takasaki H, Ohme-Takagi M
Transcriptome analyses uncover reliance of endosperm gene expression on Arabidopsis embryonic development
FEBS Letters 597: 407-417 (2023) doi: 10.1002/1873-3468.14570 - Adhikari PB, Zhu S, Liu X, Huang C, Xie L, Wu X, He J, Mitsuda N, Peters B, Brownfield L, Nagawa S, Kasahara RD
Discovery of a cis-regulatory element SaeM involved in dynamic regulation of synergid-specific MYB98
Front Plant Sci. 14 (2023) doi: 10.3389/fpls.2023.1177058 - Takasaki H, Ikeda M, Hasegawa R, Zhang Y, Sakamoto S, Maruyama D, Mitsuda N, Kinoshita T, Ohme-Takagi M
Elongation of Siliques Without Pollination 3 Regulates Nutrient Flow Necessary for Embryogenesis
Plant Cell Physiol. 64: 117-123 (2023) doi: 10.1093/pcp/pcac151 - Chen JJ, Wang W, Qin WQ, Men SZ, Li HL, Mitsuda N, Ohme-Takagi M, Wu AM
Transcription factors KNAT3 and KNAT4 are essential for integument and ovule formation in Arabidopsis
Plant Physiol. 191: 463-478 (2023) doi: 10.1093/plphys/kiac513 - Asaoka M, Sakamoto S, Gunji S, Mitsuda N, Tsukaya H, Sawa S, Hamant O, Ferjani A
Contribution of vasculature to stem integrity in Arabidopsis thaliana
Development. 13: 975 (2023) doi: 10.1242/dev.201156 - Yokoyama A, Oiwa S, Matsui T, Sawada K, Tasaka Y, Matsumura T
Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L)
Scientific Reports 12: 15951 (2022) doi: 10.1038/s41598-022-19491-z - Nosaki S, Mitsuda N, Sakamoto S, Kusubayashi K, Yamagami A, Xu YQ, Bui TBC, Terada T, Miura K, Nakano T, Tanokura M, Miyakawa T
Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout
Nat. Plants 8: 1440-1452 (2022) doi: 10.1038/s41477-022-01289-6 - Kim ED, Dorrity MW, Fitzgerald BA, Seo H, Sepuru KM, Queitsch C, Mitsuda N, Han SK, Torii KU
Dynamic chromatin accessibility deploys heterotypic cis/trans-acting factors driving stomatal cell-fate commitment
Nat. Plants. 8: 1453-1466 (2022) doi: 10.1038/s41477-022-01304-w - Zhang J, Liu Z, Sakamoto S, Mitsuda N, Ren A, Persson S, Zhang D
ETHYLENE RESPONSE FACTOR 34 promotes secondary cell wall thickening and strength of rice peduncles
Plant Physiol. 190: 1806-1820 (2022) doi: 10.1093/plphys/kiac385 - Sakamoto S, Nomura T, Kato Y, Ogita S, Mitsuda N
High-transcriptional activation ability of bamboo SECONDARY WALL NAC transcription factors is derived from C-terminal domain
Plant Biotechnology 39: 229-240 (2022) doi: 10.5511/plantbiotechnology.22.0501a - Ezura K, Nakamura A, Mitsuda N
Genome-wide characterization of the TALE homeodomain family and the KNOX-BLH interaction network in tomato
Plant Mol Biol. 109: 799-821 (2022) doi: 10.1007/s11103-022-01277-6 - Chung K, Demianski AJ, Harrison GA, Laurie-Berry N, Mitsuda N, Kunkel BN
Jasmonate Hypersensitive 3 negatively regulates both jasmonate and ethylene-mediated responses in Arabidopsis
J Exp Bot. 73: 5067-5083 (2022) doi: 10.1093/jxb/erac208 - Shimada S, Yanagawa Y, Munesada T, Horii Y, Kuriyama T, Kawashima M, Kondou Y, Yoshizumi T, Mitsuda N, Ohme-Takagi M, Makita Y, Matsui M
A collection of inducible transcription factor-glucocorticoid receptor fusion lines for functional analyses in Arabidopsis thaliana
Plant J. 111: 595-607 (2022) doi: 10.1111/tpj.15796 - Ke YD, Huang YW, Viswanath KK, Hu CC, Yeh CM, Mitsuda N, Lin NS, Hsu YH
NbNAC42 and NbZFP3 Transcription Factors Regulate the Virus Inducible NbAGO5 Promoter in Nicotiana benthamiana
Front. Plant Sci. 13 (2022) doi: 10.3389/fpls.2022.924482 - Hasegawa R, Arakawa T, Fujita K, Tanaka Y, Ookawa Z, Sakamoto S, Takasaki H, Ikeda M, Yamagami A, Mitsuda N, Nakano T, Ohme-Takagi M
Arabidopsis homeobox-leucine zipper transcription factor BRASSINOSTEROID-RELATED HOMEOBOX 3 regulates leaf greenness by suppressing BR signaling
Plant Biotechnology 39: 209-214 (2022) doi: 10.5511/plantbiotechnology.22.0128a - Hasegawa R, Fujita K, Tanaka Y, Takasaki H, Ikeda M, Yamagami A, Mitsuda N, Nakano T, Ohme-Takagi M
Arabidopsis zinc finger homeodomain transcription factor BRASSINOSTEROID-RELATED HOMEOBOX 2 acts as a positive regulator of brassinosteroid response
Plant Biotechnology 39: 185- (2022) doi: 10.5511/plantbiotechnology.22.0115a - Sekiguchi N, Sasaki K, Oshima Y, Mitsuda N
Ectopic expression of AtNF-YA6-VP16 in petals results in a novel petal phenotype in Torenia fournieri
Planta. 255: 105 (2022) doi: 10.1007/s00425-022-03876-5 - Ohta Y, Atsumi G, Yoshida C, Takahashi S, Shimizu M, Nishihara M, Nakatsuka T
Post-transcriptional gene silencing of the chalcone synthase gene CHS causes corolla lobe-specific whiting of Japanese gentian
Planta. 255: 29 (2021) doi: 10.1007/s00425-021-03815-w - Atsumi G, Matsuo K, Fukuzawa N, Matsumura T
Virus-mediated targeted DNA methylation illuminates the dynamics of methylation in an endogenous plant gene
Int J Mol Sci. 22: 4125 (2021) doi: 10.3390/ijms22084125 - Matsuo K
CRISPR/Cas9-mediated knockout of the DCL2 and DCL4 genes in Nicotiana benthamiana and its productivity of recombinant proteins
Plant Cell Rep. 41: 307-317 (2021) doi: 10.1007/s00299-021-02809-y - Matsuo K
Evaluation of methods for plant genomic DNA sequence analysis without DNA and PCR product purification
Plant Science 312: 111023 (2021) doi: 10.1016/j.plantsci.2021.111023 - Kobayashi Y, Fukuzawa N, Hyodo A, Kim H, Mashiyama S, Ogihara T, Yoshioka H, Matsuura H, Masuta C, Matsumura T, Takeshita M. Role of Salicylic Acid Glucosyltransferase in Balancing Growth and Defence for Optimum Plant Fitness. Mol. Plant Pathl., 21:429-442 (2020). DOI: 10.1111/mpp.12906
- Matsuo K. and Atsumi G., CRISPR/Cas9-mediated knockout of the RDR6 gene in Nicotiana benthamiana for efficient transient expression of recombinant proteins. Planta, 250:463-473 (2019). DOI: 10.1007/s00425-019-03180-9
- Abe, J., Wang, Y., Yamada, T., Sato, M., Ono, T., Atsumi, G., Abe, J., Hajimorad, M.R., Nakahara, K.S., Recessive resistance governed by a major quantitative trait locus restricts clover yellow vein virus in mechanically but not graft-inoculated cultivated soybeans. Mol. Plant Microbe Interact., 32:1026-1037 (2019). DOI: 10.1094/MPMI-12-18-0331-R
- Hikosaka S., Goto E., Tabayashi N., Matsumura T. Effects of air temperature before harvest on the concentration of human adiponectin in transgenic strawberry fruits. Plant Biotechnol. (Tokyo), 36:21-27 (2019). DOI: 10.5511/plantbiotechnology.19.0128a
- Matsunaga W, Shimura H, Shirakawa S, Isoda R, Inukai T, Matsumura T, Masuta C. Transcriptional silencing of 35S driven transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands. BMC Plant Biol., 19:24 (2019). DOI: 10.1186/s12870-019-1628-y
- Atsumi G., Kagaya U., Tabayashi N., Matsumura T., Analysis of the mechanisms regulating the expression of isoprenoid biosynthesis genes in hydroponically-grown Nicotiana benthamiana plants using virus-induced gene silencing. Sci. Rep. 8: 14804 (2018). DOI: 10.1038/s41598-018-32901-5
- Fukuzawa N., Masuta C., Matsumura T., Rapid transient protein production by the coat protein-deficient cucumber mosaic virus vector: non-packaged CMV system, NoPaCS. Plant Cell Rep., 37:1513-1522 (2018). DOI: 10.1007/s00299-018-2322-5
- Matsuo K. and Atsumi G., Xylosylation of proteins by expression of human xylosyltransferase 2 in plants. J. Biosci. Bioeng., 126:371-378 (2018). DOI: 10.1016/j.jbiosc.2018.03.013
- Matsuo K. and Matsumura T., Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins. J. Biosci. Bioeng., 124: 215–220 (2017). DOI: 10.1016/j.jbiosc.2017.02.019
- Atsumi G., Suzuki H., Miyashita Y., Choi S.H., Hisa Y., Rihei S., Shimada R., Jeon E.J., Abe J., Nakahara K.S., Uyeda I., P3N-PIPO, a Frameshift Product from P3, Pleiotropically Determines the Virulence of Clover Yellow Vein Virus in both Resistant and Susceptible Peas., J. Virol., 90:7388–7404 (2016).
- Miyashita Y., Atsumi G., Nakahara K.S., Trade-offs for viruses in overcoming innate immunities in plants., Mol. Plant Microbe Interact., 29:595–598 (2016).
- Yanagisawa H., Tomita R., Katsu K., Uehara T., Atsumi G., Tateda C., Kobayashi K., Sekine K.T., Combined DECS analysis and next-generation sequencing enable efficient detection of novel plant RNA viruses., Viruses 8:70 (2016).
- Tasaki K., Atsumi G., Nishihara M., Sekine K.T., Development of a Broad bean wilt virus 2-based expression vector for gentian., Sci. Hortic., 201:279–286 (2016).
- Hagiwara-Komoda Y., Choi S.H., Sato M., Atsumi G., Abe J., Fukuda J., Honjo M.N., Nagano A.J., Komoda K., Nakahara K.S., Uyeda I., Naito S., Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses. Sci. Rep., 6:21411 (2016).
- Matsuo K., Fukuzawa N., Matsumura T., A simple agroinfiltration method for transient gene expression in plant leaf discs., J. Biosci. Bioeng., 122:351–356 (2016).
- Ogawa K., Murota K., Shimura H., Furuya M., Togawa Y., Matsumura T., Masuta C., Evidence of capsaicin synthase activity of the Pun1-encoded protein and its role as a determinant of capsaicinoid accumulation in pepper. BMC Plant Biol. 28(15):93.(2015).
- Yagi H., Fukuzawa N., Tasaka Y., Matsuo K., Zhang Y., Yamaguchi T., Kondo S., Nakazawa S., Hashii N., Kawasaki N., Matsumura T., Kato K., NMR-based structural validation of therapeutic antibody produced in Nicotiana benthamiana. Plant Cell Rep. 34:959-968 (2015).
- Umali D.V., Ito A., Del Valle F.P. Shirota K. and Katoh H., Antibody response and protective immunity of chickens vaccinated with booster dose of recombinant oil-adjuvanted Leucocytozoon caulleryi subunit vaccine. Avian Dis. 58(4):609-15 (2014).
- Matsuo K., Kagaya U., Itchoda N., Tabayashi N., Matsumura T.，Deletion of plant-specific sugar residues in plant N-glycans by repression of GDP-D-mannose 4,6-dehydratase and β-1,2-xylosyltransferase genes. J. Biosci. Bioeng.，118(4):448-454 (2014).
Address in International Conference
- Itou A., Interberry alpha®: Development Process, Licensing Process and Necessary Approval, 16th Annual PEPTALK 2017, San diego, 2017/01/10
- Fukuzawa N., Ishihara T., Masuta C., Matsumura T., iral non-prolification system to produce a valuable protein in transgenic plants, 3rd International conference on Plant-Based Vaccines & Antibodies, Italy, 2009/06/16
- Matsumura T., Goto E., METI project: Development of fundamental technologies for production of high-value materials using transgenic plants in a closed-type GM plant factory, PBVA 2009, Italy, 2009/06/15
- Matsumura T., Goto E., METI project: Development of fundamental technologies for production of high-value materials using transgenic plants in a closed-type GM plant factory, PBVA 2009, Italy, 2009/06/15
- Matsumura T., Production of Pharmaceuticals in Transgenic Plants, CEAC/BIO5 Special Seminar, Arizona, 2006/12/06
- Matsumura T., Ichoda N., Ohya K., Onuma M., Sugimoto C., Protective effect of orally administered human interferon(HuINF)-α against systemic Listeria monocytogenes infection and practical advantage of HuIFN-a derived from transgenic potato plant., The 10th IAPTC&B Congress, Orland USA, 2002